9JH4

Crystal Structure of NFIA DNA-binding Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100.15
Detector technology	PIXEL
Collection date	2024-03-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	93.747, 93.747, 160.118
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.920 - 3.200
R-factor	0.2583
Rwork	0.255
R-free	0.29750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold
RMSD bond length	0.002
RMSD bond angle	0.450
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.234	1.650
Rpim	0.073	0.335
Number of reflections	12380	1759
<I/σ(I)>	29.75
Completeness [%]	100.0	100
Redundancy	24.4	24.78
CC(1/2)	0.702	0.525

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M CHES (pH 9.5), and 30% (w/v) PEG 3000