9JE9

Crystal structure of a amidase that can hydrolase PU plastic

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 43
Unit cell lengths	92.820, 92.820, 135.086
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	92.820 - 2.410
R-factor	0.2207
Rwork	0.218
R-free	0.27330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.437
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.820	2.500
High resolution limit [Å]	2.410	2.410
Number of reflections	43518	4625
<I/σ(I)>	5.5	1.1
Completeness [%]	99.0
Redundancy	2	2
CC(1/2)	0.996	0.326

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	Li2SO4, HEPES, PEG3350