9JDI

Crystal structure of the type VI secretion system effector-immunity complex BtpeA CTD-BtpiA from Bacteroides fragilis.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-03
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	113.926, 67.114, 87.374
Unit cell angles	90.00, 95.45, 90.00

Refinement procedure

Resolution	56.710 - 2.110
R-factor	0.1873
Rwork	0.186
R-free	0.21750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.489
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.710	2.180
High resolution limit [Å]	2.110	2.110
Number of reflections	36002	3772
<I/σ(I)>	13.3
Completeness [%]	94.6
Redundancy	6.1
CC(1/2)	0.997	0.556

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290.15	0.1 M Sodium Citrate (PH=5.0), 20% PEG 8000