9JDH

Crystal structure of the T6SS effector BtaeB-NTD from Bacteroides fragilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	43.670, 49.370, 183.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	91.880 - 2.180
R-factor	0.2023
Rwork	0.200
R-free	0.24820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.575
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.880	2.260
High resolution limit [Å]	2.180	2.180
Number of reflections	21523	2083
<I/σ(I)>	9.7
Completeness [%]	99.7
Redundancy	12.2
CC(1/2)	0.996	0.658

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290.15	0.1 M Sodium acetate (PH=5.2), 16% PEG3350