9JCW

Crystal structure of human MIGA1 LD targeting domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	88.645, 157.782, 163.907
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.080 - 2.600
R-factor	0.2122
Rwork	0.210
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.740
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.080	2.660
High resolution limit [Å]	2.600	2.600
Number of reflections	71396	4518
<I/σ(I)>	22.6
Completeness [%]	99.9
Redundancy	13.2
CC(1/2)	0.999	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	potassium chloride,Ammonium sulfate