9JCM

Crystal structure of Zea mays 3-phosphoglycerate dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-09
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 65 2 2
Unit cell lengths	96.336, 96.336, 503.102
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.970 - 3.490
R-factor	0.2323
Rwork	0.227
R-free	0.28640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.455
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.860	3.614
High resolution limit [Å]	3.490	3.490
Number of reflections	18410	18152
<I/σ(I)>	9.4
Completeness [%]	97.2
Redundancy	22.18
CC(1/2)	0.997	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	0.3M Sodium nitrate, 0.3M Sodium phosphate dibasic, 0.3M Ammonium sulfate, 1M Sodium HEPES, 1M MOPS (acid) pH 7.5, 40% (v/v) Glycerol, 20% (w/v) PEG 4000