9JB5

Crystal structure of Arabidopsis theliana FRATAXIN HOMOLOG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2024-05-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	52.944, 139.413, 83.292
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.210 - 2.800
R-factor	0.2197
Rwork	0.217
R-free	0.27580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.456
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.154	0.100	0.715
Rmeas	0.170	0.111	0.803
Rpim	0.072	0.048	0.358
Total number of observations	43905
Number of reflections	7924	867	751
<I/σ(I)>	4.2
Completeness [%]	99.6	99.4	97.9
Redundancy	5.5	5.1	5
CC(1/2)	0.972	0.993	0.623

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277.15	2.4 M Sodium malonate pH 7.0