9JAK

P[28] rotavirus VP8* protein with LNFP-I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-11
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	36.176, 42.660, 101.718
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.080 - 1.290
R-factor	0.2162
Rwork	0.215
R-free	0.24020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond angle	6.634
Data reduction software	autoPROC
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.860	1.380
High resolution limit [Å]	1.290	1.290
Number of reflections	33585	1680
<I/σ(I)>	15.4
Completeness [%]	92.2
Redundancy	5.1
CC(1/2)	0.999	0.584

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 mol/L KBr, 35% PEG2000MME