9JAD
GMPK in complex with GMP and K+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 65 |
| Unit cell lengths | 96.252, 96.252, 45.427 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.510 - 1.550 |
| R-factor | 0.1673 |
| Rwork | 0.166 |
| R-free | 0.20390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lvg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.004 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.130 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.074 | 0.395 |
| Rmeas | 0.077 | 0.443 |
| Rpim | 0.022 | 0.196 |
| Total number of observations | 333690 | 8222 |
| Number of reflections | 35047 | 1716 |
| <I/σ(I)> | 20.8 | 3.4 |
| Completeness [%] | 100.0 | |
| Redundancy | 9.5 | 4.8 |
| CC(1/2) | 0.998 | 0.851 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 35% PEG4000, 0.1 M Ammonium acetate, 0.1 M Trisodium citrate, pH 6.0 |
