9J8K
Crystal structure of the GluA2 ligand binding core (S1S2J) in complex with fluorophore-ligand conjugate
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 69.940, 69.940, 227.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.330 - 1.820 |
| R-factor | 0.1707 |
| Rwork | 0.169 |
| R-free | 0.19960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.078 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.330 | 1.890 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.181 | 3.369 |
| Number of reflections | 30612 | 2985 |
| <I/σ(I)> | 40.92 | 5.05 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 71.2 | 44.4 |
| CC(1/2) | 1.000 | 0.983 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1M Tris-HCl, pH8.5, 0.2M lithium sulfate, 40% v/v PEG 400 |
