9J89

zbp1 nucleic acid complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 1 21 1
Unit cell lengths	30.240, 173.950, 30.440
Unit cell angles	90.00, 94.42, 90.00

Refinement procedure

Resolution	21.570 - 1.580
R-factor	0.2136
Rwork	0.212
R-free	0.24530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.155
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21rc1_4924: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.570	1.620
High resolution limit [Å]	1.580	1.580
Rmerge	0.145	1.546
Number of reflections	42634	2831
<I/σ(I)>	9.34	1.51
Completeness [%]	99.9	99.96
Redundancy	6.2	4.9
CC(1/2)	0.995	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	ammonium sulfate