9J7J

The crystal structure of RipG4 LRR domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0000
Spacegroup name	P 65 2 2
Unit cell lengths	188.846, 188.846, 108.239
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.250 - 3.350
R-factor	0.2259
Rwork	0.224
R-free	0.26370
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.20.1-4487)
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.410
High resolution limit [Å]	3.350	3.350
Rmerge	0.097
Rmeas	0.099
Rpim	0.022
Number of reflections	16806	16806
<I/σ(I)>	28.78
Completeness [%]	99.7
Redundancy	19.9
CC(1/2)	0.997	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.1M BIS-TRIS propane pH 7.5, 1.5M ammonium tartrate dibasic