Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.473, 73.870, 123.081 |
| Unit cell angles | 90.00, 100.55, 90.00 |
Refinement procedure
| Resolution | 44.450 - 2.993 |
| R-factor | 0.2545 |
| Rwork | 0.253 |
| R-free | 0.29070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.906 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.450 | 3.100 |
| High resolution limit [Å] | 2.993 | 2.993 |
| Number of reflections | 75180 | 3859 |
| <I/σ(I)> | 8.96 | |
| Completeness [%] | 99.0 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.991 | 0.627 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.05 M Zinc acetate dihydrate, 20% w/v polyethylene glycol 3350. |
