9J70

Crystal structure of Keap1 dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	75.607, 75.627, 201.722
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.170 - 2.500
R-factor	0.1823
Rwork	0.181
R-free	0.20760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.949
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.170	2.589
High resolution limit [Å]	2.500	2.500
Number of reflections	40884	4028
<I/σ(I)>	26.26
Completeness [%]	99.9
Redundancy	13
CC(1/2)	0.999	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.05 M Zinc acetate dihydrate, 20% w/v polyethylene glycol 3350.