9J6P

pre-mir-125a internal loop in complex with G-clamp

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.605
Spacegroup name	H 3 2
Unit cell lengths	46.156, 46.156, 336.178
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.690 - 2.490
R-factor	0.2069
Rwork	0.200
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.199
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.700	39.700	2.560
High resolution limit [Å]	2.490	11.150	2.490
Rmerge	0.090	0.077	0.346
Rmeas	0.095	0.083	0.364
Number of reflections	9098	101	671
<I/σ(I)>	19.09
Completeness [%]	97.2
Redundancy	10.3
CC(1/2)	0.993	0.990	0.964

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	MPD, spermine, MOPS