9J6N
beta-D-galactofuranosidase ORF1110 from Streptomyces sp. JHA19, D-iminogalactitol complex
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-28 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.364, 57.346, 109.732 |
| Unit cell angles | 90.00, 94.93, 90.00 |
Refinement procedure
| Resolution | 47.460 - 1.700 |
| R-factor | 0.15508 |
| Rwork | 0.153 |
| R-free | 0.18934 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.697 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.460 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.097 | 0.641 |
| Rpim | 0.061 | 0.409 |
| Number of reflections | 110248 | 5375 |
| <I/σ(I)> | 9 | 1.9 |
| Completeness [%] | 98.2 | 96.8 |
| Redundancy | 3.5 | 3.4 |
| CC(1/2) | 0.995 | 0.658 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 75mM potassium thiocyanate (KSCN), 25% (w/v) PEG 3350 |
