9J6M
beta-D-galactofuranosidase ORF1110 from Streptomyces sp. JHA19, ligand-free form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-27 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.052 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.551, 57.346, 110.476 |
| Unit cell angles | 90.00, 92.05, 90.00 |
Refinement procedure
| Resolution | 47.500 - 1.800 |
| R-factor | 0.14509 |
| Rwork | 0.143 |
| R-free | 0.17661 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.728 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.500 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.088 | 0.525 |
| Rpim | 0.055 | 0.328 |
| Number of reflections | 98339 | 4807 |
| <I/σ(I)> | 9.8 | 2.6 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.991 | 0.756 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 100mM KSCN, 25% (w/v) PEG 3350 |
