9J5S

Crystal structure of human G3BP1 in complex with CHIKV nsP3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97852
Spacegroup name	C 1 2 1
Unit cell lengths	90.506, 71.450, 53.517
Unit cell angles	90.00, 95.64, 90.00

Refinement procedure

Resolution	36.190 - 2.840
R-factor	0.2289
Rwork	0.226
R-free	0.28040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fw5
RMSD bond length	0.010
RMSD bond angle	1.452
Data reduction software	MOSFLM
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.190	2.920
High resolution limit [Å]	2.840	2.850
Number of reflections	8074	605
<I/σ(I)>	4
Completeness [%]	99.9
Redundancy	6.4
CC(1/2)	0.760	0.435

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	291	20%(w/v) PEG 3350, 200mM Sodium citrate tribasic, 100mM Sodium citrate/Citric acid pH 4.0