9J50
Crystal structure of the closed state of the omega transaminase TA_5182 from Pseudomonas putida KT2440
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-03-04 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 72.570, 72.660, 106.910 |
| Unit cell angles | 95.67, 107.84, 109.19 |
Refinement procedure
| Resolution | 39.370 - 2.800 |
| R-factor | 0.24989 |
| Rwork | 0.247 |
| R-free | 0.29741 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.276 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.105 | 0.632 |
| Number of reflections | 44225 | 8374 |
| <I/σ(I)> | 5.8 | 1.36 |
| Completeness [%] | 93.7 | 95 |
| Redundancy | 1.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 295.15 | 1.4M Sodium Potassium Phosphate, pH 8.2 |
