9J4T

Structural basis for recognition of SARS-CoV-2 conserved nucleocapside epitopes by dominant T cell receptors

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-09
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	58.105, 102.725, 160.147
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.900 - 2.040
R-factor	0.18967
Rwork	0.187
R-free	0.23157
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.766
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.900	2.110
High resolution limit [Å]	2.040	2.040
Number of reflections	61913	6115
<I/σ(I)>	16.2
Completeness [%]	100.0
Redundancy	10.7
CC(1/2)	0.999	0.782

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M ammonium citrate dibasic, and 20% (w/v) PEG 3350