9J4P

Regulatory domain and kinase domain of ALPK1 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2018-02-28
Detector	AGILENT ATLAS CCD
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	93.383, 93.383, 172.270
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.010 - 2.250
R-factor	0.2077
Rwork	0.206
R-free	0.23460
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.983
Data reduction software	HKL-2000 (2.7)
Data scaling software	HKL-2000 (2.7)
Phasing software	PHASER (1.3)
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.820	2.310
High resolution limit [Å]	2.250	2.250
Number of reflections	35402	1172
<I/σ(I)>	1.64
Completeness [%]	99.0
Redundancy	2.3
CC(1/2)	1.000	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	12% PEG3350 and 0.1 M CBTP, pH 8.2, 0.1% seed