9J4K
Crystal structure of GH9l Inulinfructotransferases (IFTase) in complex with GF2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-31 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 65 |
| Unit cell lengths | 167.580, 167.580, 85.478 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.423 - 2.201 |
| Rwork | 0.146 |
| R-free | 0.18430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.433 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | RESOLVE |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.067 | 0.488 |
| Number of reflections | 69363 | 3469 |
| <I/σ(I)> | 23.48 | 2.14 |
| Completeness [%] | 100.0 | |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M lithium sulfate monohydrate 0.1 M Tris (pH 8.5) 25% (w/v) polyethylene glycol 3350 |
