9J46

Crystal structure of ASFV E146L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97918
Spacegroup name	P 65 2 2
Unit cell lengths	75.933, 75.933, 570.224
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.760 - 2.060
R-factor	0.2143
Rwork	0.212
R-free	0.25210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.999
Data reduction software	HKL-2000
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.760	2.170
High resolution limit [Å]	2.060	2.060
Rmeas	0.081
Number of reflections	52300	4553
<I/σ(I)>	0.83
Completeness [%]	83.4
Redundancy	29.9
CC(1/2)		0.869

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	5%(w/v)PEG3350,40mM Sodium Citrate PH3.9