9J3Q

Human Pigment Epithelium-Derived Factor with Zinc Ion Crystallized in P22(1)2(1) Space Group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17UM
Synchrotron site	SSRF
Beamline	BL17UM
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-27
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.25
Spacegroup name	P 21 21 2
Unit cell lengths	174.974, 61.603, 44.796
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.230 - 1.900
R-factor	0.1912
Rwork	0.189
R-free	0.23280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.961
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.740	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	72912	3819
<I/σ(I)>	35.95	0.5
Completeness [%]	99.4	99.3
Redundancy	13
CC(1/2)	0.990	0.370

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	295	0.2 M Magnesium chloride hexahydrate, 0.1 M Tris pH=8.5, 20 % w/v PEG 8000