9J32
Crystal structure of aminotransferase-like protein from Variovorax paradoxus mutant N174K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU PhotonJet-S |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-24 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.542 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 94.245, 87.340, 104.655 |
| Unit cell angles | 90.00, 99.45, 90.00 |
Refinement procedure
| Resolution | 21.810 - 2.200 |
| R-factor | 0.21714 |
| Rwork | 0.214 |
| R-free | 0.26912 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.034 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.810 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.220 | 1.380 |
| Rmeas | 0.230 | 1.430 |
| Rpim | 0.060 | 0.380 |
| Number of reflections | 42508 | 3658 |
| <I/σ(I)> | 11.7 | 1.9 |
| Completeness [%] | 99.8 | |
| Redundancy | 13.3 | 13.8 |
| CC(1/2) | 1.000 | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 12% v/v Tacsimate pH 7.0, 16% Polyethylene glycol 3350 |
