9J2K

Crystal structure of Omega Transaminase TA_2799 from Pseudomonas putida KT2440

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-02-21
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	75.000, 92.790, 136.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.310 - 1.760
R-factor	0.1468
Rwork	0.145
R-free	0.17510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.872
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.310	1.810
High resolution limit [Å]	1.760	1.760
Number of reflections	94029	6834
<I/σ(I)>	18.46
Completeness [%]	99.2	98.2
Redundancy	6.31
CC(1/2)	0.990	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.2M Ammonium acetate, 0.1M Bis-Tris pH 5.5, 2% w/v PEG 3350