9J26
Structure of a triple-helix region of human Collagen type IV from Trautec
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.99999 |
| Spacegroup name | P 1 |
| Unit cell lengths | 13.982, 30.428, 68.051 |
| Unit cell angles | 86.62, 86.47, 89.28 |
Refinement procedure
| Resolution | 33.900 - 1.450 |
| Rwork | 0.162 |
| R-free | 0.21060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k6f |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.645 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.900 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 18950 | 894 |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 95.6 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.992 | 0.980 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Index-D5:0.1M NaAc pH4.5, 25% PEG3,350 |
