9J1Y
Crystal structure of Nme1Cas9 HNH domain bound to anti-CRISPR AcrIIC1Boe.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-03 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 31 |
| Unit cell lengths | 61.753, 61.753, 50.032 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.540 - 1.790 |
| R-factor | 0.1608 |
| Rwork | 0.158 |
| R-free | 0.19060 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.687 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
| High resolution limit [Å] | 1.790 | 4.880 | 1.800 |
| Rmerge | 0.146 | 0.106 | 1.587 |
| Rmeas | 0.111 | 1.689 | |
| Rpim | 0.034 | 0.570 | |
| Number of reflections | 20145 | 1013 | 1021 |
| <I/σ(I)> | 8.1 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 10.1 | 10.4 | 8.5 |
| CC(1/2) | 0.992 | 0.731 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 289.15 | 200 nM ammonium sulfate, 100 mM sodium acetate (pH 4.5), 22% (w/v) PEG4000 |
