9J1T
Structure of a triple-helix region of human Collagen type IV from Trautec
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2024-06-25 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.99999 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 29.316, 17.717, 65.821 |
Unit cell angles | 90.00, 101.19, 90.00 |
Refinement procedure
Resolution | 32.280 - 1.450 |
Rwork | 0.190 |
R-free | 0.22040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k6f |
RMSD bond length | 0.011 |
RMSD bond angle | 1.849 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.280 | 1.470 |
High resolution limit [Å] | 1.450 | 1.450 |
Number of reflections | 11714 | 576 |
<I/σ(I)> | 17.9 | |
Completeness [%] | 96.4 | |
Redundancy | 6.7 | |
CC(1/2) | 0.998 | 0.989 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Crystal-C4:0.2M NaAc, 0.1M Sodium cacodylate pH6.5, 30% PEG8,000 |