9J1T
Structure of a triple-helix region of human Collagen type IV from Trautec
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.99999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.316, 17.717, 65.821 |
| Unit cell angles | 90.00, 101.19, 90.00 |
Refinement procedure
| Resolution | 32.280 - 1.450 |
| Rwork | 0.190 |
| R-free | 0.22040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k6f |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.849 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.280 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 11714 | 576 |
| <I/σ(I)> | 17.9 | |
| Completeness [%] | 96.4 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.998 | 0.989 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Crystal-C4:0.2M NaAc, 0.1M Sodium cacodylate pH6.5, 30% PEG8,000 |
