9J1S

Human replication protein A: RPA70 subunit N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2024-07-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97893
Spacegroup name	P 61
Unit cell lengths	57.669, 57.669, 63.122
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.580 - 1.550
R-factor	0.2216
Rwork	0.221
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b29
RMSD bond length	0.008
RMSD bond angle	1.021
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.580	1.606
High resolution limit [Å]	1.550	1.550
Rmerge	0.053
Rmeas	0.011
Number of reflections	17169	1654
<I/σ(I)>	37.98
Completeness [%]	99.0	93.6
Redundancy	2
CC(1/2)	1.000	0.557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.2 M Ammonium sulfate, 0.1 M Bis-Tris, pH 7.0, 25% PEG 3350