9J1R
Structure of a triple-helix region of human Collagen type II from Trautec
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2024-06-25 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.99999 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.777, 14.204, 48.460 |
Unit cell angles | 90.00, 105.83, 90.00 |
Refinement procedure
Resolution | 46.620 - 1.450 |
Rwork | 0.183 |
R-free | 0.22350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k6f |
RMSD bond length | 0.010 |
RMSD bond angle | 1.951 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.620 | 1.470 |
High resolution limit [Å] | 1.450 | 1.450 |
Number of reflections | 11458 | 542 |
<I/σ(I)> | 16.7 | |
Completeness [%] | 97.8 | |
Redundancy | 6.6 | |
CC(1/2) | 0.999 | 0.826 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Crystal-C7:0.2M (NH4)2SO4, 30% PEG4,000 |