9J1R

Structure of a triple-helix region of human Collagen type II from Trautec

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.99999
Spacegroup name	P 1 21 1
Unit cell lengths	47.777, 14.204, 48.460
Unit cell angles	90.00, 105.83, 90.00

Refinement procedure

Resolution	46.620 - 1.450
Rwork	0.183
R-free	0.22350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k6f
RMSD bond length	0.010
RMSD bond angle	1.951
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.620	1.470
High resolution limit [Å]	1.450	1.450
Number of reflections	11458	542
<I/σ(I)>	16.7
Completeness [%]	97.8
Redundancy	6.6
CC(1/2)	0.999	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Crystal-C7:0.2M (NH4)2SO4, 30% PEG4,000