9J1O

Mouse Spi-B Ets domain in complex with DNA containing GGAA sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-13
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	33.200, 54.870, 100.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.120 - 2.230
R-factor	0.2301
Rwork	0.228
R-free	0.27510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.867
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.290
High resolution limit [Å]	2.230	2.230
Rmerge	0.067	0.741
Number of reflections	9454	4418
<I/σ(I)>	18.06	2.3
Completeness [%]	99.7	99.9
Redundancy	6.17	6.42
CC(1/2)	0.999	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293.15	0.1M MES pH5.6, 7.6% PEG8000, 8.0% Ethylene Glycol