9J08
Acetyl xylan esterase B from Aspergillus oryzae (AoAXEB), succinate complex form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-21 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.027, 64.008, 83.324 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.100 - 1.900 |
| R-factor | 0.16545 |
| Rwork | 0.164 |
| R-free | 0.19942 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.519 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.100 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.086 | 0.219 |
| Rpim | 0.037 | 0.176 |
| Number of reflections | 23666 | 1011 |
| <I/σ(I)> | 13.7 | 4.1 |
| Completeness [%] | 96.0 | 65.8 |
| Redundancy | 5.6 | 2 |
| CC(1/2) | 0.994 | 0.715 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293.15 | 0.1 M sodium acetate (pH 4.5), 20% PEG3000 |
