9J01

Crystal Structure Sensory Appendage Protein 2 from Anopheles culicifacies

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-21
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9654
Spacegroup name	P 1 21 1
Unit cell lengths	31.365, 37.075, 41.014
Unit cell angles	90.00, 93.19, 90.00

Refinement procedure

Resolution	31.316 - 1.611
R-factor	0.1906
Rwork	0.188
R-free	0.23830
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.161
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	PHENIX ((1.15rc1_3423: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.080	1.670
High resolution limit [Å]	1.610	1.610
Rmerge	0.080	1.355
Rmeas	0.087	1.525
Rpim	0.034	0.680
Total number of observations	74827	4064
Number of reflections	12010	880
<I/σ(I)>	11.8	0.9
Completeness [%]	97.3
Redundancy	6.2	4.6
CC(1/2)	0.999	0.576

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	0.1M Lithium chloride, 0.1M Sodium acetate pH 4.5, 36% v/v PEG 400, 0.05M Cadmium chloride