9IX9
Mutant H286T Crystal Structure of Two-domain bacterial laccase from the actinobacterium Streptomyces carpinensis VKM Ac-1300
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU PhotonJet-S |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2023-04-25 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54179 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 196.546, 196.546, 196.546 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.810 - 2.050 |
| R-factor | 0.2117 |
| Rwork | 0.209 |
| R-free | 0.25830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.843 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.290 | 2.080 |
| Number of reflections | 84750 | 8403 |
| <I/σ(I)> | 8.17 | 1.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 21.1 | |
| CC(1/2) | 0.990 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 0.2M ammonium sulfate, 0.1M sodium acetate, pH 4.6, 25% v/v PEG Smear broad |
