9IWV

Crystal structure of Lsd18 after incubation with the substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1
Unit cell lengths	47.040, 61.970, 76.290
Unit cell angles	74.80, 81.86, 76.80

Refinement procedure

Resolution	32.943 - 1.850
R-factor	0.191
Rwork	0.189
R-free	0.23020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8t3p
RMSD bond length	0.007
RMSD bond angle	0.867
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.943	1.916
High resolution limit [Å]	1.850	1.850
Rmerge	0.109	1.016
Rmeas	0.118	1.096
Number of reflections	67215	4932
<I/σ(I)>	10.18	1.79
Completeness [%]	97.5	96.3
Redundancy	7.1	7.2
CC(1/2)	0.998	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291.15	0.1 M Imidazole pH 8.0, 0.8 M NaCl, 37% (w/v) PEG4000