9IW8

Crystal Structure Chemosensory Proteins 3 from Anopheles culicifacies in space group I422

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-25
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	I 4 2 2
Unit cell lengths	83.961, 83.961, 67.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.834 - 1.790
R-factor	0.2011
Rwork	0.200
R-free	0.22060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.979
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.15rc1_3423: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.834	1.820
High resolution limit [Å]	1.790	1.790
Rmeas	0.096	2.909
Number of reflections	11621	560
<I/σ(I)>	17.6	0.8
Completeness [%]	93.4	98.3
Redundancy	23.1
CC(1/2)	1.000	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1M Sodium chloride, 1.6M Ammonium sulfate, 0.1M Sodium HEPES pH 7.5