9IW6

Crystal Structure Sensory Appendage Proteins 2 from Anopheles culicifacies in space group P212121 and 2 molecule per ASU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-14
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9654
Spacegroup name	P 21 21 21
Unit cell lengths	37.443, 52.955, 114.548
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.067 - 1.427
R-factor	0.2026
Rwork	0.201
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.106
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.15rc1_3423: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.270	1.451
High resolution limit [Å]	1.427	1.427
Rmeas	0.069	1.487
Number of reflections	43303	2141
<I/σ(I)>	4	0.5
Completeness [%]	100.0	100
Redundancy	5.5	4.6
CC(1/2)	0.995	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	0.1M Lithium chloride, 0.1M Sodium acetate pH 4.5, 30% v/v PEG 400, 0.1M Cadmium chloride hemi(pentahydrate)