9IW2

Chikungunya virus E protein complexed with C37 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CMOS
Collection date	2019-07-01
Detector	RDI CMOS_8M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	57.087, 118.995, 228.048
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.850 - 3.200
R-factor	0.2246
Rwork	0.222
R-free	0.27730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.848
Data reduction software	HKL-2000
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.200	3.200
Number of reflections	26345	1693
<I/σ(I)>	3.5
Completeness [%]	99.3	64.87
Redundancy	6.1
CC(1/2)	0.966

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	HEPES, Sodium hydroxide, PEG 20000