9IVU

Crystal structure of Ang1-A451D receptor binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 64 2 2
Unit cell lengths	80.550, 80.550, 186.690
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.380 - 2.280
R-factor	0.19909
Rwork	0.196
R-free	0.24678
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.696
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.380	2.320
High resolution limit [Å]	2.280	2.280
Rmerge	0.115	1.239
Number of reflections	17164	832
<I/σ(I)>	16.4
Completeness [%]	100.0
Redundancy	9.6
CC(1/2)	0.999	0.765

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	4.0 M Sodium formate