9IVT
Crystal structure of Ang1 receptor-binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.600, 61.980, 44.550 |
| Unit cell angles | 90.00, 100.59, 90.00 |
Refinement procedure
| Resolution | 24.050 - 1.750 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.662 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.050 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.110 | |
| Number of reflections | 21020 | 1093 |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.998 | 0.775 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 3.5 M Sodium formate at pH 7.0 |
