9IV7
Crystal structure of CcmS-CcmK1-CcmK2 complex from Synechocystis sp. PCC 6803
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-20 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5406 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 133.931, 58.039, 188.768 |
| Unit cell angles | 90.00, 105.48, 90.00 |
Refinement procedure
| Resolution | 13.240 - 2.500 |
| R-factor | 0.216 |
| Rwork | 0.215 |
| R-free | 0.24060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.472 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.240 | 2.580 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.014 | |
| Rpim | 0.064 | |
| Number of reflections | 48376 | 4388 |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 98.9 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.1 M Tris (pH 8.5) and 0.7 M ammonium tartrate dibasic |
