9IV3
Crystal structure of CcmS-CcmK1 complex from Synechocystis sp. PCC 6803
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-30 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5406 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.284, 129.576, 78.272 |
| Unit cell angles | 90.00, 110.44, 90.00 |
Refinement procedure
| Resolution | 12.970 - 2.950 |
| R-factor | 0.2245 |
| Rwork | 0.222 |
| R-free | 0.27760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.408 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.970 | 3.130 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 1.689 | |
| Rpim | 0.218 | |
| Number of reflections | 24760 | 4033 |
| <I/σ(I)> | 4.9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.1 M MES (pH 6.5), 0.2 M Na acetate, and 2 M NaCl |
