9IUI

Crystal structure of PSD-95 GK domain in complex with GK_FingR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	66.429, 74.987, 136.567
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.720 - 1.930
R-factor	0.2406
Rwork	0.239
R-free	0.27820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.766
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.040
High resolution limit [Å]	1.930	5.750	1.930
Rmerge	0.138	0.035	2.402
Rmeas	0.144	0.036	2.505
Number of reflections	51565	2127	7776
<I/σ(I)>	12.82
Completeness [%]	98.7
Redundancy	13.1
CC(1/2)	0.999	1.000	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5	289	0.1M Sodium Citrate pH 5.0, 20% PEG 20000