Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-03-21 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 |
| Unit cell lengths | 115.343, 115.343, 113.362 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.260 - 2.350 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.24880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.024 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.21.1_5286_000) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 5.060 | 2.350 |
| Rmerge | 0.079 | 0.041 | 0.624 |
| Rmeas | 0.045 | 0.710 | |
| Rpim | 0.020 | 0.329 | |
| Number of reflections | 35582 | 3590 | 3516 |
| <I/σ(I)> | 14.3 | ||
| Completeness [%] | 99.8 | 98.8 | 99.9 |
| Redundancy | 4.7 | 4.7 | 4.4 |
| CC(1/2) | 0.997 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | isopropanol, Bis-Tris pH 7.1, PEG 400 |
