9IRQ
Alginate lyase (Microbulbifer sp. ALW1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978565 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.108, 90.421, 88.287 |
| Unit cell angles | 90.00, 110.01, 90.00 |
Refinement procedure
| Resolution | 82.960 - 1.980 |
| Rwork | 0.188 |
| R-free | 0.23910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nei |
| Data reduction software | XDS (1.0) |
| Data scaling software | XDS (1.0) |
| Phasing software | PHENIX (1.12) |
| Refinement software | PHENIX (1.12) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.960 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.083 | 0.525 |
| Number of reflections | 84249 | 11039 |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 94.6 | |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | Glycine ,PEG 4000, Glycine,PEG4000 |
