9IR0

phage HY126 glycosyltransferase with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	69.620, 94.970, 152.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.710 - 2.460
R-factor	0.2041
Rwork	0.203
R-free	0.21690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.135
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.670	2.520
High resolution limit [Å]	2.460	2.460
Rmerge	0.184
Number of reflections	37398	2711
<I/σ(I)>	13.7	1.6
Completeness [%]	100.0
Redundancy	12.9
CC(1/2)	0.998	0.642

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2M Ammonium acetate ; 20% w/v polyethylene glycol 3350