9IQW

phage HY126 encoded D-arabinose 1,5-diphosphate phosphatase AfhF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	96.750, 115.540, 54.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.770 - 1.640
R-factor	0.1749
Rwork	0.174
R-free	0.19070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.177
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.840	1.680
High resolution limit [Å]	1.640	1.640
Rmerge	0.068	0.868
Number of reflections	74649	4996
<I/σ(I)>	22
Completeness [%]	99.1
Redundancy	11
CC(1/2)	1.000	0.648

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2M Mg(HCOO)2.2H2O 20% PEG3350