9IQN

CatM-W86A-L6R, a SnoaL-like protein in complex with substrate mimic L6R

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-05
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	I 4 2 2
Unit cell lengths	68.485, 68.485, 126.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.140 - 2.000
R-factor	0.2292
Rwork	0.226
R-free	0.26010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	9iq5
RMSD bond length	0.011
RMSD bond angle	1.076
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.140	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	10579	901
<I/σ(I)>	10.53	2.66
Completeness [%]	93.5
Redundancy	8.3
CC(1/2)	0.996	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	100 mM tri-Sodium citrate; pH 5.0 3.15 M Ammonium sulfate