Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97851 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 52.250, 52.250, 202.839 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.710 - 1.800 |
| R-factor | 0.1987 |
| Rwork | 0.196 |
| R-free | 0.23180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 9iq7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.913 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.710 | 1.864 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 26342 | 2525 |
| <I/σ(I)> | 11.34 | |
| Completeness [%] | 97.0 | |
| Redundancy | 9.5 | |
| CC(1/2) | 0.995 | 0.889 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291.15 | 2.0 M Ammonium sulfate, 0.1 M Sodium HEPES 7.5, 2 % v/v PEG 400 |
